The effects of medical ozone on oxidative stress in lung tissue of diabetic rats

Abstract

AIM: Ozone is a trivalent form of oxygen. Medical ozone is a mixture of ozone and oxygen. Relationship between oxidant stress and diabetic complications has been shown previously. In thisstudy, it was aimed to investigate the effects of medical ozone on oxidative stress/antioxidant balance in the lung tissue of diabetic rats. METHODS: 42 adult male Sprague-Dawley rats were randomly divided into 6 groups (n=7): Control (C), ozone (O), diabetic (D), ozonetreated diabetic (DO), insulin-treated diabetic (DI), and ozone- and insulin-treated diabetic (DOI). Diabetes was induced by a single injection ofstreptozotocin (60mg/kg, i.p.), after which insulin was administered (3 IU, i.p., once a day) to the DI and DOI groups for 6 weeks, and 1.1mg/kg (50µg/ml, i.p., once a day) ozone was given to the O, DO, and DOI groups for 6 weeks. 6 weeks after the induction of diabetes, the oxidative stresstests(Total Oxidant Status, Total Antioxidant Status, Oxidative Stress Index) were made in lung tissue samples. Data were analysed using One-way ANOVA and post hoc Tukey tests. p<0.05 was considered statistically significant. RESULTS: Oxidative stress index values of D group were higher than the C, DO and DOI groups (p<0.01). There were no significant differences among the other groups in terms of oxidative stress index. CONCLUSIONS: In conclusion, medical ozone therapy alone or in combination with insulin can reduce the development of complications induced by oxidative stress in diabetic rats.