Balıkesir Üniversitesi Kurumsal Akademik Arşivi
DSpace@Balıkesir, Balıkesir Üniversitesi tarafından doğrudan ve dolaylı olarak yayınlanan; kitap, makale, tez, bildiri, rapor, araştırma verisi gibi tüm akademik kaynakları uluslararası standartlarda dijital ortamda depolar, Üniversitenin akademik performansını izlemeye aracılık eder, kaynakları uzun süreli saklar ve yayınların etkisini artırmak için telif haklarına uygun olarak Açık Erişime sunar.
Güncel Gönderiler
Determination of fermentable hidden sugars in energy drinks via crabtree-based yeast bioassay
(Namık Kemal Üniv. Tekirdağ Ziraat Fak., 2026) Yılmaz, Faysal; Dündar, Ekrem
This study aimed to evaluate the actual fermentable sugar content of a commercially available energy drink by
employing the Crabtree effect in Saccharomyces cerevisiae as a biological indicator system. To eliminate
confounding effects, caffeine was removed through liquid–liquid extraction prior to experimentation. Yeast
cultures were exposed to five different conditions: low glucose (5 g 100 mL⁻¹), high glucose (40 g 100 mL⁻¹),
labeled glucose equivalent to the beverage (11 g 100 mL⁻¹), caffeine-removed energy drink, and a glucose-free
control. Real-time monitoring of oxygen consumption, carbon dioxide release, pH dynamics, and ethanol
formation was performed using a tri-sensor fermentation monitoring system. Intracellular redox balance was
assessed through NADH/NAD⁺ quantification at 0.5 min intervals. The low-glucose group exhibited limited
oxygen depletion and moderate CO₂ production, consistent with predominantly respiratory metabolism. In contrast,
the high-glucose group showed rapid oxygen depletion and pronounced CO₂ accumulation, confirming a switch
to fermentative metabolism. The labeled-glucose condition displayed an intermediate response, characterized by
partial oxygen utilization followed by moderate fermentation. Strikingly, the caffeine-removed energy drink
induced the strongest fermentative profile, with CO₂ production reaching 91 %, pH dropping to 5.4, and ethanol
formation occurring at 2.5 min despite oxygen levels above 50 %. Furthermore, the NADH/NAD⁺ ratio in this
group increased earlier and more sharply than in all other conditions, indicating glycolytic flux exceeding
mitochondrial capacity. These findings demonstrate that the tested energy drink contained considerably higher
fermentable sugar levels than what is declared on its label. The integration of gas exchange data, pH measurements,
ethanol detection, and redox balance strongly support the applicability of the Crabtree effect as a functional
bioassay for hidden sugar detection in beverages. This approach not only complements chemical analyses but also
offers a biologically relevant method for assessing metabolic impact. From a regulatory perspective, the results
highlight the necessity for stricter labeling practices and underscore potential public health risks associated with
undeclared sugars in energy drinks.
Divergent regulation of trehalose accumulation by exogenous proline in drought-stressed sorghum and maize
(Turkish Science and Technology Publishing (TURSTEP), 2026) Genç , Tülay Turgut; Taş , Timuçin
This study investigated the effects of varying water availability and exogenous proline application on trehalose accumulation in two economically important crops, sorghum (Sorghum bicolor L.) and maize (Zea mays L.), which exhibit distinct drought tolerance strategies. The experiment was conducted under controlled conditions with four water availability levels (100%, 75%, 50% and 25%) and three proline concentrations (200, 400, and 600 mg/L). Leaf area and leaf curling were also measured to verify the successful establishment of drought stress prior to biochemical analyses. Trehalose content was determined at the V8 growth stage using a modified enzymatic assay. Results showed a reduction in leaf area with decreasing water availability and a concurrent rise in leaf curling, confirming effective induction of drought stress. Maize displayed stronger curling responses, whereas sorghum largely maintained leaf structural integrity. Regarding trehalose, sorghum naturally accumulated higher levels under severe drought stress without proline, indicating a robust innate stress response. In contrast, maize exhibited lower trehalose levels under the same conditions but showed a marked increase with proline application, suggesting a greater reliance on external osmoprotectants. Under moderate and mild stress, proline enhanced trehalose accumulation in both crops, though the response was more consistent in sorghum. Interestingly, under optimal water conditions, proline application increased trehalose levels in sorghum but had minimal effect on maize, highlighting differences in the metabolic utilization of proline. Overall, these findings underscore the importance of tailoring proline application strategies based on crop-specific stress responses and environmental conditions to optimize drought resilience. This study provides valuable insights into the complex interplay between water availability, proline, and trehalose metabolism, offering potential pathways for improving crop stress tolerance in drought-prone regions.
Early and late stage dependent antıcancer effects of laurus NOBİLİS seed extracts on pc3 prostate cancer cells
(Kocaeli Üniversitesi Kütüphane ve Dok.Dai. Başk., 2026) Hacıoğlu , Nelin
Objective: This study explored the anticancer properties of Laurus nobilis seed extracts at different maturity stages on PC3 prostate cancer cells, aiming to understand how developmental stages influence biological activities. Methods: Extracts from early-stage (green) and late-stage (black) L. nobilis seeds were tested on PC3 cells. Cytotoxicity, cell migration, wound healing, and gene expression were analyzed. HUVEC cells were used to assess toxicity on normal cells. Results: Early-stage extracts showed low cytotoxicity and promoted cell migration and wound healing. Late-stage extracts exhibited potent dose-dependent cytotoxicity and significantly inhibited cell migration. Both extracts demonstrated lower toxicity towards HUVEC cells. Gene expression analysis revealed suppression of epithelial-mesenchymal transition and migration in cells treated with late-stage extracts. Conclusion: The study highlights the stage-dependent anticancer potential of L. nobilis seed extracts. Late-stage extracts showed promising anticancer effects, while early-stage extracts promoted cell growth. These findings emphasize the importance of harvest time and maturity stage in determining the biological activity of L. nobilis extracts, providing valuable insights for future anticancer research and applications.
The effect of the individualised instruction model on students' attitudes towards physical education and sport: Experimental study
(Tokat Gaziosmanpaşa Üniversitesi, Lisansüstü Eğitim Enstitüsü, 2026) İrhan, Gökhan; Sivrikaya, Ahmet Haktan
The aim of this study is to examine the effect of the individualised teaching model on students' attitudes towards physical education and sports. A pre-test and post-test control group model, one of the full experimental models, was used in the research. At the beginning of the study, after matching the groups, 30 students (15 in the experimental group and 15 in the control group) in the 9th grade formed the study group through random assignment. The lessons were conducted over 6 weeks using the basketball unit; the students in the experimental group were taught using the individualised teaching model, while the students in the control group were taught using the direct teaching model. Data collection utilised a unidimensional "Attitude Scale Towards Physical Education and Sports" consisting of 24 items. Data analysis was performed using a 2x2 repeated measures analysis of variance following preliminary analyses. The findings of the study revealed a significant Group x Measurement (Pre-Test/Post-Test) interaction effect. Significant differences were determined in the pre-test and post-test scores of the experimental and control groups. The findings of the retention test revealed that the positive attitude continued for the experimental group, while the negative attitude towards physical education and sports continued for the control group. The results of the study revealed that the individualised teaching model positively affected the attitudes of first-year high school students towards physical education and sports. No findings were obtained indicating that lessons taught using the direct teaching model developed students' attitudes towards physical education and sports. Physical education and sports teachers may be advised to use the individualised teaching model in their lessons to develop attitudes towards physical education and sports.
Human health oriented design approaches in office buildings: Salutogenic model
(Mehmet Topçu, 2026) Kutluer , Eda Nur Aydemir; Bakır , Betül
Office buildings have frequently changed from the past to the present due to historical factors (the collapse of empires, colonialism and expansionist policies, the industrial revolution, wars, the development of technology, epidemics, etc.) and the transformations these factors have created in user needs. Office designs, based on user health and productivity, have also had to keep pace with the changing needs of the age. Therefore, the design of office spaces is an important criterion that reflects how people feel and how it affects their work performance. Research examining the relationship between health and office environments often focuses on a single variable (noise, lighting, office layout, air quality, etc.) and addresses the pathogenic effects of the physical environment, while largely ignoring salutogenic components. Thus, there is a need for a theoretical framework and a holistic approach that encompasses health-promoting research data by adopting the concept of “salutogenic,” introduced by sociologist Aaron Antonovsky, which aims to address the origins of health and promote well-being in a positive manner. The aim of this article is to explore how the adoption of salutogenic principles in the architectural design of offices can positively affect the physical, mental, and social health of employees and how this process should be managed. The data obtained through a literature review method enable the application of salutogenic models in office buildings. Within the scope of this study, Antonovsky’s concept of the salutogenic model is examined, and the adaptation of design models developed based on this concept is discussed in the context of office buildings.
