What are the mechanisms mediating the neuroprotective effects of ozone in diabetic neuropathy?

Abstract

AIM: Ozone, which hastherapeutic effect in many pathologies, partially prevented diabetic neuropathy in ratsin our previousstudy. In the present study, it was investigated that the mechanisms mediating the neuroprotective effects of ozone in diabetic neuropathy.mly divided into 6 groups (n=7): control (C), ozone (O), diabetic (D), ozone-treated diabetic (DO), insulin-treated diabetic (DI), and ozone- and insulin-treated diabetic (DOI). Diabetes was induced by a single injection of streptozotocin (60mg/kg, i.p.), after which insulin was administered (3 IU, i.p., once a day) to the DI and DOI groups for 6 weeks, and 1.1mg/kg (50µg/ml, i.p., once a day) ozone was given to the O, DO, and DOI groups for 6 weeks. 6 weeks after the induction of diabetes, the electrophysiological, biochemical and histopathological tests were made. Data were analysed using the one way ANOVA and post hoc Tukey tests. p<0.05 was considered statistically significant. RESULTS: While the blood glucose, HbA1c, plasma total oxidantstatus, oxidative stressindex and apoptotic cell number of the D group were significantly higher than the C and O groups, the same values of DO, DI and DOI groups were significantly lower than the D group. While the nerve conduction velocity, amplitude of compound action potential, axon number and endoneural capillary number of the D group were significantly lower than the C and O groups, the same values of DO, DI and DOI groups were significantly higher than the D group. CONCLUSIONS: Consistent with previous studies, ozone reduced the blood glucose and HbA1c levels, and partially prevented diabetic neuropathy in this study. These findings indicate that the neuroprotective effect of ozone in diabetic neuropathy are mediated through oxidative stress, vascular and apoptotic mechanisms. We believe that ozone, as a potential therapeutic agent for diabetic neuropathy, a focus on ozone and a molecule to be investigated in detail