Effects of epidermal growth factor (egf) on caıx and caxıı expression ın mg-63 cells

Abstract

Carbonic anhydrases catalyse the reversible reaction from H2Oand CO2to HCO3-ions. Carbonic Anhydrase IX and CarbonicAnhydrase XII are member of the carbonic anhydrase family.Carbonic Anhydrase IX and Carbonic Anhydrase XII are in cellmembrane. Carbonic Anhydrase IX expressed in solid tumor cell.CAIX expression in many cancer types is associated with diseaseprocesses. Elucidation of CAIX regulation associated with cancerwould be a new approach in cancer diagnosis. Epidermal growthfactor (EGF) is a growth factor that stimulates cell growth, pro-liferation, and differentiation by binding to its receptor EGFR.Human EGF is a 6045-Da protein with 53 amino acid residuesand three intramolecular disulfide bonds. The aim of this studywas to investigate the CA IX and CA XII mRNA expression lev-els in osteosarcoma cells (MG-63) treated with EGF. Therefore,MG-63 cells were treated by EGF for different time intervals,namely 1h, 3h, 6h, 24h, 48h and 72h. EGF upregulates CA IXmRNA expression mainly at 1h, 3h and 6h. EGF upregulatesCA XII mRNA expression mainly at 3h and 6h. Different con-centration of EGF on CAIX cells were also investigated andoptimum dose for maximum expression was determined.