Quantification of main secondary metabolites of Satureja icarica P.H. Davis (Lamiaceae) by LC-HRMS and evaluation of antioxidant capacities

Abstract

In this study, we determined antioxidant activity of Satureja icarica (Lamiaceae) which is used as a spice by the local population against 1,1-diphenyl-2-picryl-hydrazyl (DPPH·), N,N-dimethyl-p-phenylenediamine radicals (DMPD•+), and 2,2-azino-bis-3-ethylbenzthiazoline-6-sulfonic acid (ABTS•+) as well as their ability to reduce Fe3+, Fe3+ -TPTZ, and Cu2+ . The antioxidant activities of both extracts were similar to those of standards in all assays. For instance, IC50 of S. icarica were for scavenging DPPH was 10.19 μg/mL. The results of liquid chromatography-high resolution mass spectrometry (LC-HRMS) revealed that the main secondary metabolites in Satureja icarica were found to be as rosmarinic acid and luteolin-7-O-rutinoside.