Comparison of hydratase, esterase activities and inhibition manner of mutant Asn67/Ile and Leu204/Ser of human CAII

Abstract

Carbonic anhydrases (CAs; also known as carbonate dehydratases EC 4.2.1.1) are a family of enzymes which catalyze the reversible reaction from H2O and CO2 to HCO3 - ions. Carbonic anhydrases are ubiquitous metalloenzymes present in prokaryotes and eukaryotes that are encoded by five evolutionarily unrelated gene families, namely a- CAs, b-CAs, c-CAs, d-CAs and e-CAs. In mammals, 15 a-CA isozymes or CA-related proteins have been described with different catalytic activity, subcellular localization and tissue distribution.