The effect of some pesticides on acetylcholinesterase activity from bovine braine

Abstract

Acetylcholinesterase (AChE) inhibitors are widely used for a variety of medical, agricultural and public health purposes. Consequently, exposure is highly possible during lifetime. Therefore, the aim of this study was to investigate in vitro the effects of pesticides such as cypermethrin, fenpropathrin, 1,1-D-methylpiperidinium chloride, N-(phosphonomethyl)gylcine, beta-naphthoxyacetic acid, lambdacyhalothrin, deltamethrin, and 2,4-dichoroplenoxy acetic acid on AChE activity obtained from bovine braine. Pesticides showed different effects on AChE activity. The AChE enzyme was inhibited by cypermethrin, fenpropathrin, 1,1-D-methylpiperidinium chloride, N-(phosphonomethyl)gylcine, and beta-naphthoxyacetic acid pesticides and activated by lambdacyhalothrin and deltamethrin pesticides. 2,4-dichoroplenoxy acetic acid pesticide did not alter the activity of AChE enzyme. AChE activity was inhibited in a concentration-dependent manner, and calculated IC50 values for cypermethrin (IC50=24.45 mM), fenpropathrin (IC50=16.95 mM), 1,1-D-methylpiperidinium chloride (IC50=69.09 mM), N-(phosphonomethyl)gylcine (IC50=585 mM), and beta-naphthoxyacetic acid (IC50=16.34 mM).