Effect of Epidermal Growth Factor on Osteosarcoma Cell Proliferation and Bcl-2 Gene Expression

Abstract

Objective: The aim of this study was to investigate the effects of epidermal growth factor (EGF) on osteosarcoma cell proliferation and Bcl-2 expression.Methods: In this study, MTT test was first applied to determine the effect of EGF on cell proliferation. Twenty ng/?L EGF was added to the Saos-2 cell line and the effectson cell proliferation were determined after different incubation intervals. At the next experimental stage, 20 ng/mL EGF was added to the osteosarcoma cell line andthe expression of antiapoptotic Bcl-2 was evaluated at 1-, 3-, 24-, 48- and 72-hour.Results: A statistically significant increase in cell proliferation was observed at 3-, 24- and 72-hour after administration of 20 ng/?L EGF in the osteosarcoma cell line.EGF was observed to stimulate Bcl-2 expression at a dose of 20 ng/mL at 1- and 24-hour.Conclusion: EGF; at the 3rd, 24th and 72nd hours, a statistically significant increase in cell proliferation was observed. In addition, the expression of antiapoptotic Bcl-2was evaluated at the 1st, 3rd, 24th, 48th and 72th hours on the EGF osteosarcoma cell line. The expression of Bcl-2 was increased at the end of the 1st and 24th hours ofapplication of EGF at 20 ng/mL dose.