Detection of apoptosis in experimental fibrosarcoma using DNA fragmentation and immunohystochemical methods

Abstract

Fibrosarcoma is driving from mesenchimal tissue. It is one of the malign tumors and usually seen in cats and dogs however, it can be encountered in all animals. Apoptosis used for eliminating of cells which are functioning not properly. Recent studies on tumors showed that there is a connection between cancers and apoptosis. In this study, apoptosis in fibrosarcomas induced by 3-methylcholanthrene using immunuhystochemical and ELISA Methods was investigated. In order to induce fibrosarcoma in rats at the beginning of the experiment animals were injected subcutaneously on the neck with 0.2 mg 3-methylcholanthrene solved in 0.25 mL sesame oil. During the experiment took between 150-210 days depending on the appearance of tumor tissue. At the end of the experiment animals were killed under the ether anesthesia and necropsy is performed. DNA fragments of tumor tissue cell were analized using ELISA, localisation of Bcl-2 and Bax was determined by immunohystochemical method, Immunohistochemically there was a lot of Bcl-2 and Bax positive cells. Contrary to this in control animals there was rarely. Furthermore, the absorbance of DNA fragmentation of experimental, animals was 4 times higher than that of controls. This data indicates that in the tumor tissues it is observed some cells programmed to death by apoptosis but the others not.