The effects of ex vivo ozone treatment on erythrocyte carbonic anhydrase enzyme

Abstract

AIM: Medical ozone generates beneficial effects by stimulating the endogenous antioxidant systems. Carbonic anhydrases (CAs, EC 4.2.1.1.) are metalloenzymes and play a significant role in acid-base regulation. The aim of this study was to investigate the effects of medical ozone on human red blood cell CA (hCA) enzyme activity. METHODS: Human blood samples were treated with different doses of ozone (10, 20, 30 μg/ml) and the erythrocyte total CA enzyme activities were determined. Also, purified hCA I and hCA II isozymes were treated with same doses of ozone and enzyme activities were measured. The hCA I and II enzymes activities were assayed by following the hydration of CO2 according to the method described by Wilbur and Anderson (1976). One-way analysis of variance (ANOVA) and post hoc Tukey test were used to compare the experimental groups. p<0.05 was considered significant.RESULTS: There was no statistically significant alteration in activity of purified hCA I and hCA II treated with ozone compared to untreated control. Total CA activity of 30 μg/ml ozone-treated group was significantly higher than the 10 μg/ml ozone-treated group (p<0.05). CONCLUSIONS:The ex vivo ozone doses used in this study did not cause any negative effect on erythrocyte CA enzyme activity.