A new method for purification of carbonic anhydrase isozymes by affinity chromatography
| dc.contributor.author | Özensoy, Özen | |
| dc.contributor.author | Arslan, Oktay | |
| dc.contributor.author | Sinan, Selma Öznür | |
| dc.date.accessioned | 2019-10-17T07:08:58Z | |
| dc.date.available | 2019-10-17T07:08:58Z | |
| dc.date.issued | 2004 | en_US |
| dc.department | Fakülteler, Fen-Edebiyat Fakültesi, Kimya Bölümü | en_US |
| dc.department | Fakülteler, Fen-Edebiyat Fakültesi, Biyoloji Bölümü | en_US |
| dc.description.abstract | A new affinity gel for purification of carbonic anhydrase isozymes was prepared using EUPERGIT(R) C-250L derivatized with p-aminobenzenesulfonamide, an inhibitor of carbonic anhydrase. The binding capacity of the affinity gel was determined at different temperatures, pH values, elution buffers, and ionic strengths. Human carbonic anhydrase isozymes (HCA I and HCA II) and bovine carbonic anhydrase (BCA) were purified in high yields from erythrocytes. | en_US |
| dc.identifier.doi | 10.1023/B:BIRY.0000018954.38006.9a | |
| dc.identifier.endpage | 219 | en_US |
| dc.identifier.issn | 0006-2979 | |
| dc.identifier.issn | 1608-3040 | |
| dc.identifier.issue | 2 | en_US |
| dc.identifier.scopus | 2-s2.0-3543097391 | |
| dc.identifier.scopusquality | Q2 | |
| dc.identifier.startpage | 216 | en_US |
| dc.identifier.uri | https://doi.org/10.1023/B:BIRY.0000018954.38006.9a | |
| dc.identifier.uri | https://hdl.handle.net/20.500.12462/7505 | |
| dc.identifier.volume | 69 | en_US |
| dc.identifier.wos | WOS:000220754400014 | |
| dc.identifier.wosquality | Q4 | |
| dc.indekslendigikaynak | Web of Science | |
| dc.indekslendigikaynak | Scopus | |
| dc.language.iso | en | en_US |
| dc.publisher | Maik Nauka/Interperiodica/Springer | en_US |
| dc.relation.ispartof | Biochemistry-Moscow | en_US |
| dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
| dc.rights | info:eu-repo/semantics/openAccess | en_US |
| dc.subject | EUPERGIT(R) C-250L | en_US |
| dc.subject | Carbonic Anhydrase | en_US |
| dc.subject | Affinity Chromatography | en_US |
| dc.title | A new method for purification of carbonic anhydrase isozymes by affinity chromatography | en_US |
| dc.type | Article | en_US |
