In vitro inhibition of the carbonic anhydrase from saanen goat (Capra hircus) with pesticides

Abstract

This study was conducted to determine the in vitro effects of four commonly used pesticides (Nuarimol(Tm), Fenarimol(TM), Parathionmethyl(Tm) and 2,4-D-TM) on erythrocyte carbonic anhydrase (CA) activity from Saanen goats (SG). The enzyme was 262.57-fold purified by affinity chromatography and the purity was confirmed by SDS-PAGE. Inhibitory effect of the pesticides on the purified enzyme was determined using the CO2-hydratase activity method. IC50 values of the pesticides that caused inhibition were determined by means of activity percentage diagrams. The concentrations of Nuarimol(TM), Fenarimol(TM), and 2,4-DTM that inhibited 50% of the enzymatic activity were 0.352, 0.924 and 2.04 mM, respectively. Conversely, the enzyme activity was increased by parathion-methyl.