Effect of ellagic acid on some oxidative stress parameters and cyclooxygenase-2 reactivity in mice with experimental gastric injury

Abstract

Non-steroidal anti-inflammatory drugs (NSAIDs) commonly used by people induces gastric injury. This study was designed to evaluate effects of ellagic acid as a natural compound on oxidative stress markers and immunohistochemical structure during experimental gastric damage. It was created five groups as follows. Group I was received standard pellet feed and drinking water. On the other hand, a single oral dose of 25 mg/kg indomethacin, 10 mg/kg ellagic acid, 25 mg/kg indomethacin plus 10 mg/kg ellagic acid and 20 mg/kg omeprazole were received to group II, III, IV and V, respectively. The reduced glutathione (GSH), nitric oxide (NO), malondialdehyde (MDA), total sialic acid (TSA) and ghrelin levels of samples taken after 6 hours from applications were analyzed by spectrophotometric methods. The cyclooxygenase 2 (COX-2) reactivity was analyzed by immunohistochemical staining method. The kidney NO and TSA levels of group II were found to be increased compared with group I, whereas these levels were lower in group IV compared with group II. The liver NO and MDA levels of group II were higher than in group IV. All blood ghrelin levels in group II were lower compared with other groups. It was revealed severe COX-2 immunoreactivity in stomach surface and foveola epithelium, parietal cells, macrophages and vascular endothelium near submucosa of group II, while this reactivity was less in group IV. It was concluded that ellagic acid significantly changed NO, TSA, MDA and ghrelin levels and stomach COX-2 activity of mice given indomethacin and ellagic acid prevented gastric injury.