Effects of some pesticides on purified human paraoxonase 1 activity, in vitro

Abstract

Using pesticides in agriculture is a significant problem for the environment and humans. Human paraoxonase 1 (hPON1) is an antioxidant enzyme associated with HDLs in the blood that hydrolyses some pesticides like paraoxon. Its activity is essential for human health because of pathophysiological phrases such as coronary artery diseases (CADs), stroke, Parkinson's disease, changes in total plasma levels, and LDL. Many heavy metals, some pesticides, even COVID 19 are responsible for reducing the enzyme's activity. In this study, hPON 1 was purified from human serum blood with two steps: ammonium sulfate precipitation and hydrophobic gel formulated as Sepharose 4B L-tyrosine 1-naphthyl amine. After the purification, inhibition studies were started using carbaryl, carbofuran, coumatetralyl, dazomet, amitraz, tebuconazole, dimethachlor, dichlofluanid, simazine, 1-naphthol, alachlor, and propoxur are used as pesticides. They all showed inhibition effects on purified hPON1 enzyme. IC50 values were determined to be in the range of 9.24 (+/- 0.63) to 17.98 mu M (+/- 1.04). K-i values were determined to be in the range of 7.15 to 14.55 mu M.