Determination of venom components from the endoparasitoid wasp Pimpla turionellae L. (Hymenoptera : Ichneumonidae)

dc.contributor.authorUçkan, Fevzi
dc.contributor.authorSinan, Selma
dc.contributor.authorSavaşçı, Şahin
dc.contributor.authorErgin, Ekrem
dc.date.accessioned2019-10-17T07:17:37Z
dc.date.available2019-10-17T07:17:37Z
dc.date.issued2004en_US
dc.departmentFakülteler, Fen-Edebiyat Fakültesi, Biyoloji Bölümüen_US
dc.descriptionUçkan, Fevzi (Balikesir Author)en_US
dc.description.abstractVenom from the endoparasitoid wasp Pimpla turionellae L. (Hymenoptera; Ichneumonidae) was isolated in pure form. Total protein determination indicated an average value of 0.04 mug protein per venom sac. The molecular weights of the venom components were estimated with reference to molecular weight markers and reference proteins by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Analysis indicated that venom primarily contains proteins with molecular weights between 20 and 106 kDa. The presence of melittin and apamin in wasp venom was shown by SDS-PAGE and reversed-phase high-performance liquid chromatography (HPLC). Infrared spectroscopic data confirmed the acidic nature of the venom and the presence of amines, peptides, proteins, and enzymes in the venom. Venom noradrenaline was separated using thin-layer chromatography and verified by infrared spectroscopy.en_US
dc.identifier.doi10.1603/0013-8746(2004)097[0775:DOVCFT]2.0.CO;2
dc.identifier.endpage780en_US
dc.identifier.issue4en_US
dc.identifier.scopus2-s2.0-4344657568
dc.identifier.scopusqualityQ1
dc.identifier.startpage775en_US
dc.identifier.urihttps://doi.org/10.1603/0013-8746(2004)097[0775:DOVCFT]2.0.CO;2
dc.identifier.urihttps://hdl.handle.net/20.500.12462/7596
dc.identifier.volume97en_US
dc.identifier.wosWOS:000222642400021
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoenen_US
dc.publisherEntomol Soc Ameren_US
dc.relation.ispartofAnnals of The Entomological Society of Americaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/embargoedAccessen_US
dc.subjectPimpla Turionellaeen_US
dc.subjectVenomen_US
dc.subjectElectrophoresisen_US
dc.subjectChromatographyen_US
dc.subjectInfrared Spectroscopyen_US
dc.titleDetermination of venom components from the endoparasitoid wasp Pimpla turionellae L. (Hymenoptera : Ichneumonidae)en_US
dc.typeArticleen_US

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