Agents isolated from horses with respiratory system infection signs

Abstract

In this study, it was aimed to isolate the agents and to determine the antibiotic susceptibility of the agents by taking nasal swabs from horses with respiratory system problems. Nasal swab samples were taken from 30 horses of different age groups with respiratory system symptoms in South Marmara Region. The samples were cultured on Blood agar, MacConkey agar, Mycoplasma agar and, Sabouraud Dextrose agar. Colony morphology, gram staining and biochemical tests were performed for identification. Disk diffusion method was used to determine the antimicrobial susceptibility of the agents. Ten different microorganism species were isolated from nasal swabs taken from horses of different ages with respiratory system problems. As a result of the biochemical tests, 9 Streptococcus zooepidemicus (27.27%), 4 Staphylococcus aureus (12.12%), 4 Coagulase-negative staphylococci (CoNS) (12.12%), 4 Klebsiella pneumoniae (12.12%), 3 Pasteurella spp. (9.09%), 2 Streptococcus pyogenes (6.06%), 2 Bacillus spp. (6.06%), 2 Corynebacterium spp. (6.06%), 2 Candida spp. (6.06%) and 1 Rhodococcus equi (3.03%) were identified. The most Streptococcus zooepidemicus (27.27%) and the least Rhodococcus equi (3.03%) were identified from nasal swab samples. The most sensitive antibiotic of the isolates was Amoxicillin / clavulanic acid (96.87%), and the most resistant was Penicillin-G (18.75%).Bu çalışmada solunum sistemi sorunu olan atlardan nasal svaplar alınarak etkenlerin izolasyonu ve antibiyotik duyarlılıklarının belirlenmesi amaçlanmıştır. Güney Marmara Bölgesi’nde solunum sistemi semptomları olan farklı yaş gruplarındaki 30 attan nasal svap örnekleri alındı. Örnekler Blood agar, MacConkey agar, Mycoplasma agar ve Sabouraud Dextrose agarda kültüre edildi. İdentifikasyon için koloni morfolojisi incelemesi, gram boyama ve biyokimyasal testler yapıldı. Etkenlerin antimikrobiyal duyarlılıklarını belirlemek için disk difüzyon yöntemi kullanıldı. Solunum sistemi sorunları olan farklı yaşlardaki atlardan alınan nasal svaplardan 10 farklı mikroorganizma türü izole edildi. Biyokimyasal testler sonucunda 9 Streptococcus zooepidemicus (%27,27), 4 Staphylococcus aureus (%12,12), 4 Koagülaz negatif stafilokok (KNS) (%12,12), 4 Klebsiella pneumoniae (%12,12), 3 Pasteurella spp. (%9.09), 2 Streptococcus pyogenes (%6.06), 2 Bacillus spp. (%6.06), 2 Corynebacterium spp. (%6.06), 2 Candida spp. (%6.06) ve 1 Rhodococcus equi (%3.03) tespit edildi. Nasal svap örneklerinden en fazla Streptococcus zooepidemicus (%27.27) ve en az Rhodococcus equi (%3.03) izole edildi. İzolatların en duyarlı olduğu antibiyotik Amoksisilin/klavulanik asit (%96.87), en dirençli olduğu ise Penisilin-G (%18.75) idi.