An investigation on the determination of diurnal and ontogenetic variations of essential oil composition in sideritis trojana growing in kazdagi (Edremit-Balikesir)

Abstract

This study was conducted to determine the essential oil composition and diurnal variations (9.00 am, 12.00 am and 16.00 pm) of Sideritis trojana Bornm. which was distributed naturally at the peak of Kazdagi. (Edremit-Balikesir), west. of Turkey during the growing season. The oil composition was determined with GC-MS. The highest, quantity of essential oil content. (0.25-0.30%) was found at the full plant flowering stage and the lowest was at fresh fruiting stage (0.07-0.1%). Diurnal fluctuation in essential oils of whole plant was also observed. The highest and lowest, essential oil levels were observed at the afternoon (16.00 pm) and in the morning (08.00 am). respectively. The oils were consisted of mainly a-bisabolol (27.8%), valeranone (13.4%). 4-terpineol (10.3%), germacrene-D (8.8%) and spathulenol (5.8%) during vegetative stage, 4-terpineol (30.3%), caryophyllene (21.0%) and 3-methyl nonane (9.3%) during flowering stage, 4-terpineol (16.1%), copaene (15.4%), caryophyllene (10.6%), 3-methyl nonane (9.8%) and valeranone (7.9%) during fresh fruiting stage.Bu araştırmada, Kazdağları’nda (Edremit-Balıkesir) yayılış gösteren Sideritis trojana Bornm türünün uçucu yağ oranı ve bileşenlerinin diurnal ve ontogenetik varyasyonunun belirlenmesi amaçlanmıştır. Uçucu yağ bileşenleri GC-MS analizi ile yapılmıştır. En yüksek uçucu yağ oranı çiçeklenme döneminde (%0.25-30), en düşük uçucu yağ oranı ise meyve döneminde %0.07-0.10) tespit edilmiştir. Uçucu yağ bileşenleri vejetatif dönemde esas olarak; α-bisabolol (%27.8), valeranone (%13.4), 4-terpineol (% 0.3), germacrene-D (%8.8) ve spathulenol (%5.8); çiçeklenme döneminde ise 4-terpineol (% 30.3), caryophyllene (%21.0) ve 3-methyl nonane (9.3%); meyve oluşumu döneminde 4-terpineol (%16.1), copaene (% 15.4), caryophyllene (%10.6), 3-methyl nonane (%9.8) ve valeranone (%7.9) olarak belirlenmiştir.