Preventive effect of selenium pretreatment against cisplatin- induced oral mucositis in rats

Abstract

Objectives. The aim of the present study was to investigate the protective role of selenium against cisplatininduced oral mucositis in rats. Methods. Healthy wistar albino rats (n=21) were randomly divided into threegroups: Cisplatin (Cis), cisplatin and selenium (Cis+Se) and control (C). Cisplatin was administered for 3 daysto Cis and Cis+Se groups. Cis+Se group received selenium 5 days before cisplatin injection and continued for11 consecutive days. Malondialdehyde (MDA) levels of the rats were determined before and at the end ofexperimental protocol. The tongues of rats were harvested for immunuhistochemical examinations. Results.In biochemichal analysis, MDA levels significantly increased in the Cis group (p=0.018). On the contrary,there was no significant difference between pretreatment and posttreatment MDA levels in Cis+Se group(p=0.128). In the immunohistochemical examinations of lingual tissues stained with anti-caspase-3, when theCis and Cis+Se groups were compared, there were significantly more immunopositivity in the Cis group andsignificantly less immunopositivity in the Cis+Se group (p<0.05). Conclusions. Selenium might have playeda protective role against cisplatin-induced oral mucositis in rats. Further studies are necessary before its clinicaluse in patientsObjectives. The aim of the present study was to investigate the protective role of selenium against cisplatininduced oral mucositis in rats. Methods. Healthy wistar albino rats (n=21) were randomly divided into threegroups: Cisplatin (Cis), cisplatin and selenium (Cis+Se) and control (C). Cisplatin was administered for 3 daysto Cis and Cis+Se groups. Cis+Se group received selenium 5 days before cisplatin injection and continued for11 consecutive days. Malondialdehyde (MDA) levels of the rats were determined before and at the end ofexperimental protocol. The tongues of rats were harvested for immunuhistochemical examinations. Results.In biochemichal analysis, MDA levels significantly increased in the Cis group (p=0.018). On the contrary,there was no significant difference between pretreatment and posttreatment MDA levels in Cis+Se group(p=0.128). In the immunohistochemical examinations of lingual tissues stained with anti-caspase-3, when theCis and Cis+Se groups were compared, there were significantly more immunopositivity in the Cis group andsignificantly less immunopositivity in the Cis+Se group (p<0.05). Conclusions. Selenium might have playeda protective role against cisplatin-induced oral mucositis in rats. Further studies are necessary before its clinicaluse in patients