The effects of cardiac drugs on human erythrocyte carbonic anhydrase I and II isozymes

Abstract

Cardiovascular diseases are the leading cause of mortality worldwide. In recent years, the relationship between carbonic anhydrase inhibitors and atherosclerosis has attracted attention. In this study, we aimed to determine thein vitroeffects of 35 frequently used cardiac drugs on human carbonic anhydrase I (hCA I) and II (hCA II). The inhibitory effects of the drugs on hCA I and hCA II were determined with both the hydratase and esterase methods. The most potent inhibitors observed were propafenone (hCA I: 2.8 mu M and hCA II: 3.02 mu M) and captopril (hCA I: 1.58 mu M and hCA II: 6.25 mu M). Isosorbide mononitrate, propranolol, furosemide, and atorvastatin were also potent inhibitors. The inhibitor constant,K-i, value from the Lineweaver-Burk plot for propafenone was 2.38 mu M for hCA I and 2.97 mu M for hCA II. The tested cardiac drugs showed potentin vitroinhibition of the hCA I and II isozymes. Especially, in patients with atherosclerotic heart disease, these drugs may be preferred primarily due to the beneficial effects of carbonic anhydrase inhibition on atherosclerosis.