In vitro effects of some antibiotics on enzyme activity of carbonic anhydrase from bovine erythrocytes

Abstract

The purpose of this study was to investigate the in vitro effects of six commonly used veterinary medical drugs (Killoxacin (TM), Gentavet (TM), Geosol (TM), Primoxal (TM), Lypectin (TM) and Penoksal-la (TM)) on erythrocyte bovine carbonic anhydrase (CA, EC 4.2.1.1) activity. The enzyme was purified by affinity chromatography, and the purity was confirmed by SDS-PAGE. Inhibition or activation effects of six different antibiotics on the purified enzyme were determined using the CO2-hydratase activity method. IC50 values of the drugs that caused inhibition were determined by means of Activity % vs [Inhibitor] diagrams. The concentrations of Killoxacin (TM), Gentavet (TM), Geosol (TM), Primoxal (TM) and Lypectin (TM) that inhibited 50% of the enzymatic activity were 9.25, 3.79, 0.72, 6.59 and 2.12 mM, respectively. On the other hand, the enzyme activity was increased by Penoksal-la (TM).