80 günlük yaban domuzu fötüslerinde ön ve arka extremitelerin kemikleşme ve gelişimleri

Abstract

GİRİŞ ve AMAÇOBJECTIVE: Having information about the normal skeletogenous stages is essential in order to comment on experimental bone development studies. With this regard, we aimed at revealing the normal ossification status of the 32 extremities of the 80 days old wild pig fetuses. METHODS: The materials were fixed in 5% formaldehyde solution and 95% ethanol, followed by pure acetone incubation. Then, samples were stained with an alcian blue-alizarin red combination. As a result of the staining procedure, the cartilaginous tissue was stained in blue and the osseous tissue was stained in red. By using this method, it is possible to display the development of the cartilaginous components and localization of the early centers of the ossification areas in the bones. Chondrification and ossification of the bones were observed attentively under a stereo microscopy. RESULTS: Each big bone has only one primary ossification body center (POC). The ossification centers in all bones have been shown whereas there are no ossification centers in the carpal, tarsal bones and patella. The extremities with the blue stained cartilaginous and red osseous tissues have resembled to the gross structural shapes of the mature stages. DISCUSSION AND CONCLUSION: Only one secondary ossification center (SOC) was observed in the distal part of radius of one specimen.