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dc.contributor.authorGençer, Nahit
dc.contributor.authorArslan, Oktay
dc.date.accessioned2019-10-21T08:50:03Z
dc.date.available2019-10-21T08:50:03Z
dc.date.issued2009en_US
dc.identifier.issn1570-0232
dc.identifier.urihttps://doi.org/10.1016/j.jchromb.2008.11.037
dc.identifier.urihttps://hdl.handle.net/20.500.12462/9089
dc.description.abstractIn this study, a new purification strategy for human PON1 enzyme was developed using two-step procedures, namely ammonium sulfate precipitation and sepharose-4B-L-tyrosine-9-aminophenantrene hydrophobic interaction chromatography. SDS polyacrylamide gel electrophoresis of the enzyme indicates a single band with an apparent MW of 43 kDa. Overall purification rate of our method was found 901-fold for R isoenzyme and 453-fold for Q isoenzyme. The V-max and K-M of the purified enzyme were determined for Q isoenzyme 55 EU and 0.599 mM and for R isoenzyme 50 EU and 0.492 mM, respectively. The in vitro effects of some heavy metals (Hg, Cd, Cu, Mn and Ni) were investigated on the purified human serum PON1Q and R isoenzyme. using paraoxon as substrate. Metals were more effective inhibitors on purified human serum PON1(R192) activity than PON1(QI92) activity. The kinetics of interaction of metals with the purified human serum PON1(R192) and PON1(Q192) indicated a different inhibition pattern. Kinetic constants K-M, V-max, and inhibition type were determined. (c) 2008 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipBalikesir University 2007/11en_US
dc.language.isoengen_US
dc.publisherElsevier Science Bven_US
dc.relation.isversionof10.1016/j.jchromb.2008.11.037en_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectPON1en_US
dc.subjectHydrophobic Interaction Chromatographyen_US
dc.subjectPurificationen_US
dc.subjectPhenotypeen_US
dc.subjectMetalsen_US
dc.subjectInhibitionen_US
dc.titlePurification human PON1Q192 and PON1R192 isoenzymes by hydrophobic interaction chromatography and investigation of the inhibition by metalsen_US
dc.typearticleen_US
dc.relation.journalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciencesen_US
dc.contributor.departmentFen Edebiyat Fakültesien_US
dc.contributor.authorID0000-0001-7092-8857en_US
dc.identifier.volume877en_US
dc.identifier.issue3en_US
dc.identifier.startpage134en_US
dc.identifier.endpage140en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


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