Ovine PAPPA2 gene coding variants are linked to decreased fecal egg shedding in native Turkish sheep naturally infected with gastrointestinal nematodes
Özet
In this study, the association between PAPPA2 coding variants and gastrointestinal (GI) nematode fecal egg count (FEC)
score in adult Turkish sheep was investigated. For this purpose, the FEC score was determined in adult sheep from six
breeds: Karacabey Merino (n=137), Kivircik (n=116), Cine capari (n=109), Karakacan (n=102), Imroz (n=73), and
Chios (n=50). Sheep were classifed as shedders or non-shedders within breeds and focks. The frst group was the fecal egg
shedders (>50 per gram of feces), and the second group was the no fecal egg shedders (≤50 per gram of feces). The exon
1, exon 2, exon 5, exon 7, and a part of 5′UTR of the ovine PAPPA2 gene were genotyped by Sanger sequencing of these
two groups. Fourteen synonymous and three non-synonymous single-nucleotide polymorphisms (SNPs) were found. The
non-synonymous SNPs, D109N, D391H, and L409R variants, are reported for the frst time. Two haplotype blocks were
constructed on exon 2 and exon 7. The specifc haplotype, C391G424G449T473C515A542 on the exon 2 that carries the 391H
variant, was tested against four other common haplotypes. Our results indicate that C391G424G449T473C515A542 haplotype was
signifcantly associated with fecal egg shedding status in adult Turkish sheep (p-value, 0.044).
