Inhibitory effect on acetylcholinesterase and toxicity analysis of some medicinal plants
Özet
This study aimed to analyse the inhibition of different extracts of
Rosmarinus officinalis, Pistacia terebinthus and Sideritis dichotoma on
acetylcholinesterase enzyme of Drosophila melanogaster. Additionally, the
biological features including antioxidant activity, phenolic contents, antibacterial
effects and in vivo toxicities were identified using radical scavenging, Folin Ciocalteu, disc diffusion methods, and larval (eclosion) assay using Drosophila,
respectively. Also, GC-MS was used to determine of the terpene-derivative
compositions of the plants. IC50 values on acetylcholinesterase were determined
between 0.57±0.02-2.54±0.11µg µL-1
for ethanol, 0.86±0.05-2.19±0.15µg µL-1
for methanol and 1.98±0.13-4.76±0.24µg µL-1
for water extracts. Inhibition types
of Rosmarinus, Pistacia and Sideritis were uncompetitive, competitive and
competitive, respectively. The antioxidant activities of the extracts were between
77.87±1.72-96.94±1.84% against DPPH and 90.57±2.18-98.18±2.36% against
ABTS+
radicals. GC/MS results showed that carvacrol and thymol were the major
monoterpenes of Pistacia and Sideritis, while limonene and borneol were the
main monoterpenes of Rosmarinus. The strongest antibacterial activities were
observed with Rosmarinus and Sideritis against Staphylococcus aureus and
Escherichia coli, respectively with an inhibition zone larger than 15 mm.
According to the in vivo toxicity study, all extracts were found non-toxic to
Drosophila, and they ameliorated H2O2 induced decrease of puparation, survival
rate and eclosion values.
