Yazar "Alper, Meltem" için listeleme
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Amplification of GC-rich ADAMTS-2 and URG4/URGCP promoter regions with optimized combination of PCR enhancers
Alper, Meltem; Tokay, Esra; Koçkar, Feray (Tubitak Scientific & Technical Research Council Turkey, 2016)PCR is an effective and widely used method for the amplification of target DNA fragments in vitro. The templates that have high guanine-cytosine (GC) content, complex secondary structures, or short tandem repeats are ... -
Cloning of human ADAMTS-2 Promoter: Strategies for cloning extremely GC rich promoters
Alper, Meltem; Köçkar,Feray (Wiley-Blackwell, 2011)ADAMTS (A Disintegrin and Metalloproteinase with Thrombo-spondin Motifs) are zinc dependent proteases have a part in impor-tant physiologic processes such as development, homeostasis andfertility. To date, 19 different ... -
Effects of fibroblast growth factor (FGF acidic) and epidermal growth factor (EGF) on procollagen-N proteinases; ADAMTS-2 and ADAMTS-3 in osteosarcoma model
Alper, Meltem; Köçkar, Feray (Wiley-Blackwell, 2012)A Disintegrin and Metalloproteinase domain, with Thrombo-Spondin (ADAMTS) is a family of zinc-dependent proteases thathave common features with A Disintegrin and Metalloproteinase(ADAM) and also MMP enzymes (Matrix ... -
Evaluation of in vitro effects of some analgesic drugs on erythrocyte and recombinant carbonic anhydrase I and II
Gökçe, Başak; Gençer, Nahit; Arslan, Oktay; Türkoğlu, Sümeyye Aydoğan; Alper, Meltem; Köçkar, Feray (Informa Healthcare, 2012)The in vitro effects of the injectable form of analgesic drugs, dexketoprofen trometamol, dexamethasone sodium phosphate, metamizole sodium, diclofenac sodium, thiocolchicoside, on the activity of purified human carbonic ... -
Expression analysis of URG4 (upregulated gene 4) in osteoblast-like cells, Saos-2 and MG63
Köçkar, Feray; Tokay, Esra; Alper, Meltem (Wiley-Blackwell, 2011)URG4 is a novel gene firstly found in uninfected liver cells stimu-lated by HBxAg (Hepatitis B virus encoded X antigen) and HCC(Hepatocellular Carcinoma) cells. It is associated with cellgrowth, growth factor-independent ... -
Expression pattern of adamts-3 (A disintegrin and matrix metalloproteinase type,1 motif 3) in normal and cancer cell lines
Alper, Meltem; Aydemir, A. Tuğşen; Köçkar, Feray (2022)ADAMTS-3 is a procollagen N- proteinase whose main function is to cleave procollagen II, the principal collagen of the cartilage and bone. This maturation process provides accurate fibril conformation for tissues. Along ... -
Hypoxic regulation of ADAMTS-2 and-3 (a disintegrin and matrix metalloproteinase with thrombospondin motifs 2 and 3) procollagen N proteinases by HIF-1 alpha in endothelial cells
Altuntaş, Candan; Alper, Meltem; Keleş, Yasemin; Sav, Feyza Nur; Köçkar, Feray (Springer, 2023)ADAMTS-2 and ADAMTS-3, known as procollagen amino proteases (PNP), are primarily responsible for processing the amino ends of the fbrillar collagen precursors. ADAMTS-2 is a highly expressed gene in type I collagen-rich ... -
IL-6 upregulates a disintegrin and metalloproteinase with thrombospondin motifs 2 (ADAMTS-2) in human osteosarcoma cells mediated by JNK pathwa
Alper, Meltem; Köçkar, Feray (Springer, 2014)ADAMTS-2 and ADAMTS-3 (a disintegrin and metalloproteinase with thrombospondin type 1 motif 2) belong to the procollagen aminoproteinase subfamily of ADAMTS proteases. They play crucial roles in the collagen metabolism. ... -
IL-6’nın ADAMTS-8 gen ifadesi üzerine etkisinin belirlenmesi
Kötüz, Nuriye (Balıkesir Üniversitesi Fen Bilimleri Enstitüsü, 2022)Kolorektal kanser dünya çapında en sık görülen kanserlerden biridir. ADAMTS-8 önemli bir anjiyoinhibitör gendir. IL-6 sitokinin kolorektal kanser ile ilişkili olduğunu gösteren çalışmalar mevcuttur. Bu tezin amacı, IL-6 ... -
Induction of human ADAMTS-2 gene expression by IL-1 alpha is mediated by a multiple crosstalk of MEK/JNK and PI3K pathways in osteoblast like cells
Alper, Meltem; Aydemir, Tuğşen; Köçkar, Feray (Elsevier Science BV, 2015)Up-regulation of ADAMTS genes with proinflammatory cytokines is important for some pathological conditions such as osteoarthritis (OA) that is a disease based on ECM degradation in cartilage. IL-1 alpha is a proinflammatory ... -
İnsan ADAMTS2 geninin transkripsiyonel regülasyonu
Alper, Meltem (Balıkesir Üniversitesi Fen Bilimleri Enstitüsü, 2013)ADAMTS'ler (A Disintegrin And Metalloprotease domain with ThromboSpondin type I motifs) embriyonik gelişim, anjiyogenez ve kıkırdak degredasyonu gibi önemli fizyolojik süreçlerde görev yapan metaloproteazlardır. Bu ailenin ... -
Molecular characterization of zeta class glutathione s-transferases from pinus brutia ten.
Öztetik, Elif; Köçkar, Feray; Alper, Meltem; İşcan, Mehmet (Indian Acad Sciences, 2015)Glutathione transferases (GSTs; EC 2.5.1.18) play important roles in stress tolerance and metabolic detoxification in plants. In higher plants, studies on GSTs have focussed largely on agricultural plants. There is restricted ... -
Molecular cloning and biochemical characterization of a Tau class glutathione S-transferase from Pinus brutia Ten
Alper, Meltem; Öztetik, Elif; Kaya, Mihrap Yaşar; Köçkar, Feray (Springer Heidelberg, 2020)Key message A new Tau class GST gene was cloned from Pinus brutia Ten. cDNA sequence was analysed for conserved sequences. Substrate specificity, optimum pH, and temperature values of the recombinant PbGST Tau enzyme were ... -
Mutation of active site residues Asn67 to Ile, Gln92 to Val and Leu204 to Ser in human carbonic anhydrase ii: influences on the catalytic activity and affinity for inhibitors
Türkoğlu, Sümeyye; Maresca, Alfonso; Alper, Meltem; Köçkar, Feray; Işık, Semra; Sinan, Selma; Özensoy, Özen; Arslan, Oktay (Pergamon-Elsevier Science Ltd, 2012)Site-directed mutagenesis has been used to change three amino acid residues involved in the binding of inhibitors (Asn67Ile; Gln92Val and Leu204Ser) within the active site of human carbonic anhydrase (CA, EC 4.2.1.1) II ... -
SP1-mediated downregulation of ADAMTS3 gene expression in osteosarcoma models
Alper, Meltem; Aydemir, Ayşe Tugşen; Köçkar, Feray (Elsevier Science, 2018)ADAMTS3 is a member of procollagen N-proteinase subfamily of ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) gene family. It has an important function in the procollagen maturation process. The ...
